Anmol Chemicals is the pioneer manufacturers of Methylene Blue, Pharmaceutical Excipients Food & Flavor chemicals in India. We offer Halal and Kosher Methylene Blue made in an ISO9001, ISO22000 (FSSC22000) and cGMP certified facility. Our group has several manufacturing facilities spread across the world, supported by toll manufacturers and representatives in UAE, Europe, USA, China and has several associated manufacturing facilities spread across India. All the Information on Physics, Chemistry, Applications, Uses and Technology on Manufacture of Methylene Blue is in these pages.
The units have one or more of the certifications like FDA GMP, ISO 9001, ISO 22000, HACCP, REACH, Kosher & Halal

Home ---------------- Contact

Drum Packing Palletized Drum Packing Palletized & Shrink Wrapped. 25 50kg HDPE Laminated Bags. Exports to USA UAE Europe Tanzania Kenya Egypt Turkey Brazil Chile Argentina Dubai Methylthioninium Chloride Methylene Blue Manufacturers

This is a summary computer translation of our web page. For Details and Safety Data Sheet of Methylthioninium Chloride, Methylene Blue Manufacturers, please visit our Home Page.

Methylthioninium Chloride, Methylene Blue Manufacturers

Methylene Blue is also called Basic blue 9 (CI 52015) - 3,7-Bis-(dimethylamino)-phenazathonium chloride - 3,7-Bis-(dimethylamino)-5-phenothiazinium chloride - Methylene blue (CI 52015) - Methylthionine chloride - Solvent blue 8 (CI 52015) - Swiss blue - Tetramethylthionine chloride - Urolene blue

CAS Number 61-73-4 (Anhydrous); 7220-79-3 (Trihydrate)

Specifications of Commercial Grade Methylene Blue are as follows:
Chemical Formula: C16H18N3CIS
Molecular Weight: 319.85 gm / mole
Chemical Name: 3,7-bis(Dimethylamino)-phenazathionium chloride Tetramethylthionine chloride
CAS Number 61-73-4
Appearance: Dark Green Crystalline Powder
Assay (as is basis): 80 % - 85%
Assay (on dry basis): 98% -101%
Loss on drying: 10% -20%
Solubility: Clear Blue Solution
Ash content: 1% maximum
Residual Solvent: Nil
Aluminium: 100 ppm maximum
Cadmium: 1ppm maximum
Chromium: 10ppm maximum
Copper: 50ppm maximum
Tin: 10 ppm maximum
Iron: 100ppm maximum
Mangenese: 10ppm maximum
Mercury: Nil
Molybdenum: 10ppm maximum


Methylene Blue USP
C16H18ClN3S - 3H2O -- 373.90
Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride, trihydrate.
C.I. Basic Blue 9 trihydrate [CAS Number 7220-79-3]
Anhydrous 319.86 [CAS Number 61-73-4]

Methylene Blue contains not less than 98.0 percent and not more than 103.0 percent of C16H18ClN3S, calculated on the dried basis.

Identification: Infrared Absorption

Loss on drying: Dry it at 75 and at a pressure not exceeding 5 mm of mercury for 4 hours: it loses between 8.0% and 18.0% of its weight.
Residue on ignition: not more than 1.2%.
Arsenic: Prepare the Test Preparation by mixing 0.375 g with 10 mL of water in the arsine generator flask. Add 15 mL of nitric acid and 5 mL of perchloric acid, mix and heat cautiously to the production of strong fumes of perchloric acid. Cool, wash down the sides of the flask with water and again heat to strong fumes. Again cool, wash down the sides of the flask and heat to fumes. Cool, dilute with water to 52 mL and add 3 mL of hydrochloric acid: the resulting solution meets the requirements of the test, the addition of 20 mL of 7 N sulfuric acid specified for Procedure being omitted. The limit is 8 ppm.
Copper or zinc: Ignite 1.0 g in a porcelain crucible, using as low a temperature as practicable, until all of the carbon is oxidized. Cool the residue, add 15 mL of 2 N nitric acid, and boil for 5 minutes. Filter the cooled solution, and wash any residue with 10 mL of water. To the combined filtrate and washing add an excess of 6 N ammonium hydroxide, and filter the solution into a 50 mL volumetric flask. Wash the precipitate with small portions of water, adding the washings to the filtrate, dilute the solution with water to volume, and mix. To 25 mL of the solution add 10 mL of hydrogen sulfide TS: no turbidity is produced within 5 minutes (absence of zinc). Any dark color produced does not exceed that of a control prepared by boiling a quantity of cupric sulfate, equivalent to 200 @g of copper, with 15 mL of 2 N nitric acid for 5 minutes and by treating this solution as directed above, beginning with “Filter the cooled solution” (0.02% of copper).
Chromatographic purity: Quantitatively dissolve an accurately weighed quantity of Methylene Blue in methanol to obtain a Test solution containing 1.0 mg per mL Dissolve a suitable quantity of USP Methylene Blue in methanol to obtain a Standard solution having a concentration of 100 @g per mL. Quantitatively dilute a portion of this solution with methanol to obtain a Diluted standard solution having a concentration of 10 @g per mL. Apply 5 @L each of the Test solution, the Standard solution, and the Diluted standard solution to a suitable thin-layer chromatographic plate coated with a 0.25-mm layer of octadecylsilanized chromatographic silica gel. Allow the spots to dry, and develop the chromatogram in a chromatographic chamber with a solvent system consisting of a mixture of the upper layer separated from a well-shaken mixture of water, n-butanol, and glacial acetic acid (100:80:20), until the solvent front has moved about three-fourths of the length of the plate. Remove the plate from the chamber, allow the solvent to evaporate, and visually locate the spots on the plate: the RF value of the principal spot in the chromatogram from the Test solution corresponds to that from the Standard solution, and other spots, if present in the chromatogram from the Test solution, consist of a secondary spot that does not exceed in size or intensity, the principal spot obtained from the Standard solution (10%), and not more than two additional spots, neither of which exceeds in size or intensity the principal spot from the Diluted standard solution (1%)

Methylthioninium Chloride BP or Methylthioninium Chloride Ph Eur is also supplied.

Exports to USA, Canada, UAE, Dubai, South Africa, Tanzania, Kenya, Nigeria, Egypt, Uganda, Turkey, Mexico, Brazil, Chile, Argentina, Europe Netherlands, Italy, Spain, Germany, Portugal, France, Malaysia, Indonesia, Thailand, Vietnam, Korea, Japan, etc.

Methylene Blue USP Methyl Thioninium Chloride Manufacturers:

Anmol Chemicals
S-8, SARIFA MANSION, 2ND FLANK ROAD, CHINCHBUNDER, MUMBAI 400009, INDIA
TEL: (OFFICE) 91-22-23770100, 23726950, 23774610, 23723564 - FAX: 91-22-23728264

e-mail: anmolc@mtnl.net.in

Anmol Chemicals India

Copyright and Usual Disclaimer is Applicable. 25 January, 2022